Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions / 南方医科大学学报
Journal of Southern Medical University
; (12): 418-420, 2007.
Article
em Zh
| WPRIM
| ID: wpr-268118
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis.</p><p><b>METHODS</b>Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting.</p><p><b>RESULTS</b>Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis.</p><p><b>CONCLUSION</b>The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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RNA Mensageiro
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Condiloma Acuminado
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Regulação para Baixo
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Regulação da Expressão Gênica
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Western Blotting
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Receptores CXCR4
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Análise de Sequência com Séries de Oligonucleotídeos
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Epiderme
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Quimiocina CCL20
Limite:
Adult
/
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Article