Interaction between a novel centrosomal protein TACP1 and mitotic kinase Nek2A / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 337-342, 2007.
Article
em Zh
| WPRIM
| ID: wpr-271525
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study interaction between a novel centrosomal protein TACP1 and mitotic kinase Nek2A.</p><p><b>METHODS</b>Nek2A305-446 protein was expressed and purified in E.coli and TACP1 protein was expressed in transfected 293T cells. Pull-down assay was used to examine the interaction between Nek2A305-446 and TACP1. TACP1 and Nek2A complex was tested by co-immunoprecipitation assay with polyclonal anti-TACP1 antibody. The localization of those two proteins in Hela cells was verified by immunofluorescence.</p><p><b>RESULTS</b>TACP1 was pulled down by Nek2A305-446 protein but not by GST control. Nek2A was co-precipitated with TACP1 protein by polyclonal anti-TACP1 antibody but not by pre-immunization serum. The Immunofluorescence test showed that these two proteins formed a complex at centrosome during mitosis.</p><p><b>CONCLUSION</b>Centrosomal protein TACP1 is a novel interacting protein with Nek2A, both of which are localized in centrosome during mitosis.</p>
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Assunto principal:
Ligação Proteica
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Proteínas Recombinantes
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Células HeLa
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Transfecção
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Linhagem Celular
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Imunofluorescência
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Proteínas Serina-Treonina Quinases
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Centrossomo
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Proteínas de Ligação a Telômeros
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Imunoprecipitação
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2007
Tipo de documento:
Article