AntiEGFRnano inhibites proliferation and migration of estrogen-dependent Ishikawa cells of human endometrial cancer cell line / 药学学报
Acta Pharmaceutica Sinica
; (12): 1341-1346, 2012.
Article
em Zh
| WPRIM
| ID: wpr-274656
Biblioteca responsável:
WPRO
ABSTRACT
Nanobody is a kind of antibody from camel, which misses light chain. Nanobody has the same antigen binding specificity and affinity as mAb. Moreover, because of its small molecular weight, high stability and easy preparation, nanobody has great value of biomedical applications. In this study, we successfully prepared highly pure antiEGFR nanobody in E.coli using genetic engineering techniques. Cell proliferation assay (CCK-8 assay) and migration experiments (cell scratch test and Transwell assay) indicated that the recombinant antiEGFRnano can significantly inhibit the proliferation and migration of endometrial cancer cells. These results provide a new way of thinking and methods for EGFR-targeted therapy of endometrial cancer.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Plasmídeos
/
Proteínas Recombinantes
/
Sequência de Bases
/
Adenocarcinoma
/
Movimento Celular
/
Sequência de Aminoácidos
/
Neoplasias do Endométrio
/
Linhagem Celular Tumoral
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2012
Tipo de documento:
Article