DNA Vaccines Encoding Toxoplasma gondii Cathepsin C 1 Induce Protection against Toxoplasmosis in Mice
The Korean Journal of Parasitology
; : 505-512, 2017.
Article
em En
| WPRIM
| ID: wpr-27500
Biblioteca responsável:
WPRO
ABSTRACT
Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant α-GalCer. As results, TgCPC1 DNA vaccine with or without adjuvant α-GalCer showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and IFN-γ in the spleen compared to controls (PBS, pEGFP-C1, and α-Galcer). Upon challenge infection with tachyzoites of T. gondii (RH), pCPC1/α-Galcer immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and α-Galcer). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Peptídeo Hidrolases
/
Baço
/
Toxoplasma
/
DNA
/
Imunoglobulina G
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Linfócitos B
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Catepsinas
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Toxoplasmose
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Interleucina-2
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Epitopos de Linfócito T
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2017
Tipo de documento:
Article