Ten significantly differentially expressed genes in prostate cancer: Screening and verification / 中华男科学杂志
National Journal of Andrology
; (12): 408-413, 2015.
Article
em Zh
| WPRIM
| ID: wpr-276084
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and verify differentially expressed genes in prostate cancer.</p><p><b>METHODS</b>Using DNA microarray, we screened differentially expressed genes in prostate cancer tissue and its adjacent tissue followed by verification by PCR.</p><p><b>RESULTS</b>A total of 1 444 genes were found to be differentially expressed (differentiation ≥ 1.5-fold; P≤ 0.05) in the prostate cancer tissue, of which 769 (53%) were up-regulated and 675 (47%) down-regulated. Fifty percent of the differentially expressed genes showed a 1.5- to 2-fold differentiation, including 396 up-regulated and 182 down-regulated ones. Additionally, 308 up-regulated and 334 down-regulated genes exhibited a >2- to 5-fold, 46 up-regulated and 78 down-regulated genes a > 5- to 10-fold, and 19 up-regulated and 81 down-regulated genes a > 10-fold differentiation. Verification by subjecting 15 most significantly up-regulated and another 15 most markedly down-regulated genes to quantitative real-time PCR (qRT-PCR) showed that most of the genes had a transcriptional profile similar to that in the microarray data, with a Pearson correction coefficient of 0.83 between the microarray data and qRT-PCR results. Totally, 10 significantly differentially expressed genes were identified.</p><p><b>CONCLUSION</b>DNA microarray analysis provides reliable information on differentially expressed genes in prostate cancer and benign tissues. The 10 significantly differentially expressed genes verified by qRT-PCR could possibly become new bio-markers and specific molecules for tumor identification.</p>
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WPRIM
Assunto principal:
Neoplasias da Próstata
/
Regulação para Baixo
/
Expressão Gênica
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Regulação Neoplásica da Expressão Gênica
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Diferenciação Celular
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Ativação Transcricional
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Regulação para Cima
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Reação em Cadeia da Polimerase
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Análise de Sequência com Séries de Oligonucleotídeos
/
Genética
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2015
Tipo de documento:
Article