Selection and identification of salt-tolerant variants of Taraxacum officinale / 生物工程学报
Chinese Journal of Biotechnology
; (12): 262-271, 2008.
Article
em Zh
| WPRIM
| ID: wpr-276129
Biblioteca responsável:
WPRO
ABSTRACT
In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
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Índice:
WPRIM
Assunto principal:
Farmacologia
/
Superóxido Dismutase
/
Variação Genética
/
Cloreto de Sódio
/
Folhas de Planta
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Técnica de Amplificação ao Acaso de DNA Polimórfico
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Taraxacum
/
Plântula
/
Flavonas
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Técnicas de Cultura
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Article