Studies on TLC fingerprint of flavonoids in rhizome of Polygonatum odoratum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control.</p><p><b>METHOD</b>The ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid (5:4:1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultraviolet light (365 nm). The plate was scanned at wavelengths of lambdaR = 500 nm, lambdaS = 280 nm.</p><p><b>RESULT</b>A fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up.</p><p><b>CONCLUSION</b>The method can be used for quality control of P. odoratum.</p>