Apoptosis of HL-60 cells induced by artesunate in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 506-509, 2007.
Article
em Zh
| WPRIM
| ID: wpr-276884
Biblioteca responsável:
WPRO
ABSTRACT
The study was aimed to investigate the effect of artesunate (ART) on the apoptosis of HL-60 cells in vitro and the expression of Bcl-2 and ICAD in the process of apoptosis induced by ART. The inhibition of ART on HL-60 cells were evaluated by means of MTT assay; cell apoptosis was detected by light microscopy, agarose gel electro-phoresis, flow cytometry; Western blot was used to analyze the expression of Bcl-2 and ICAD in cells during apoptosis induced by ART. The results showed that ART could significantly inhibit the proliferation of HL-60 cells in time-and dose-dependent manner. After treating HL-60 cells with ART for 48 hours, the IC(50) values was 18.33 microg/ml and its inhibition effect contributed to the induced apoptosis. Bcl-2 and ICAD proteins both all expressed in HL-60 cells, the level of expression declined as concentration increased. It is concluded that artesunate may induce apoptosis of HL-60 cells in vitro, Bcl-2 and ICAD may be an important control factor in the signal transduction pathway of ART-induced apoptosis.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Farmacologia
/
Apoptose
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Células HL-60
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Proteínas Proto-Oncogênicas c-bcl-2
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Artemisininas
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Proteínas Reguladoras de Apoptose
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Metabolismo
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Article