The role of p53 binding site on the trans binding of p53 to Hsp90 beta gene / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 285-288, 2002.
Article
em Zh
| WPRIM
| ID: wpr-278181
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of p53 binding site (+31/+60) of hsp90 beta gene on its transcriptional regulation.</p><p><b>METHODS</b>The binding site was first inserted into pBS-SK. After the plasmid annealing and elongation with mutagenic and selective primers, nuclease digestion and bacteria transformation was performed twice to select the positive mutated plasmid. Electrophoretic mobility shift assays (EMSA) was employed to detect the binding of hsp90 beta gene fragment containing mutated p53 binding site and Jurkat cell nuclear extract transfected by p53 expression vector.</p><p><b>RESULTS</b>The sequence analysis profile confirmed a successful mutation of two bases on the core sequence of the second half binding site. EMSA results showed the specific DNA-protein complex band disappeared after the mutation.</p><p><b>CONCLUSIONS</b>The core sequence of p53 binding site plays a key role in the trans binding of p53 to hsp90 beta gene.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Transcrição Gênica
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Sítios de Ligação
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Leucemia de Células T
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Ativação Transcricional
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Mutagênese Sítio-Dirigida
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Proteína Supressora de Tumor p53
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Proteínas de Choque Térmico HSP90
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Genética
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2002
Tipo de documento:
Article