Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study / 南方医科大学学报
Journal of Southern Medical University
; (12): 722-724, 2008.
Article
em Zh
| WPRIM
| ID: wpr-280111
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the function of F10 gene, a novel hydaditiform mole-related gene.</p><p><b>METHODS</b>A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).</p><p><b>RESULTS</b>The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.</p><p><b>CONCLUSION</b>F10 gene is functionally related to cell proliferation and apoptosis.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oncogenes
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Patologia
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Neoplasias Uterinas
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Transfecção
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Mola Hidatiforme
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Regulação Neoplásica da Expressão Gênica
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Apoptose
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Mola Hidatiforme Invasiva
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Perfilação da Expressão Gênica
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Linhagem Celular Tumoral
Limite:
Female
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Humans
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Pregnancy
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Article