Variation of 4 kinds of compounds in Psoralea corylifolia processed by different methods / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2071-2075, 2011.
Article
em Zh
| WPRIM
| ID: wpr-283255
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WPRO
ABSTRACT
<p><b>UNLABELLED</b>OBJECITVE To established an efficient method for simultaneous quantification of 7 compounds belonging to 4 chemical types in Psoralea corylifolia processed by different methods, and to elucidate variations of 4 kinds of compounds in different processed P. corylifolia.</p><p><b>METHOD</b>The chromatographic separation was performed on an ACUITY C18 column using acetonitrile and 0.1% formic acid aqueous solution in the gradient elution at 0.4 mL x min(-1). Detection wavelength was set at 246 nm. Column temperature was fixed at 50 degrees C.</p><p><b>RESULT</b>The 4 kinds of compounds including psoralenoside, isopsoralenoside (benzofuran glycosides), psoralen, isopsoralen, psoralidin (coumarins), bavachin (flavonoids), and bakuchiol (meroterpenes) were separated in 25 min. The correlation coefficients of those compounds were over 0.9993 in the tested range. The intra- and inter-day precisions were below 1.5%. The average recoveries ranged from 99.2% to 106%.</p><p><b>CONCLUSION</b>This method is simple, rapid and accurate, which can be used for the determination of the 4 kinds of compounds in different processed P. corylifolia. In P. corylifolia processed by different methods, the contents of benzofuran glycosides, flavonoids, and meroterpenes in P. corylifolia processed by Leigong's method decrease obviously, while the contents of psoralen and isopsoralen increase significantly.</p>
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Assunto principal:
Plantas Medicinais
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Química
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Reprodutibilidade dos Testes
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Cromatografia Líquida de Alta Pressão
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Tecnologia Farmacêutica
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Psoralea
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Estabilidade de Medicamentos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Article