Expression changes of age-related genes in different aging stages of Caenorhabiditis elegans and the regulating effects of Chuanxiong extract / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1599-1602, 2010.
Article
em Zh
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| ID: wpr-285319
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ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression changes of age-related genes in different stages of aging and the regulating effects of Chuanxiong extract on it.</p><p><b>METHOD</b>According to the different stages of aging, the experiments were tested at two time points of 2 d and 6 d. Using realtime RT-PCR (qRT-PCR) to test the expression change of aging-related genes among the groups.</p><p><b>RESULT</b>Compared with the 2 d control group, the expression of age-1, daf-2, let-363 were up-regulated in the 6 d control group (P < 0.05) while the expression of ins-18, let-60, sir-2.1, sod-3 were down-regulated (P < 0.05). Compared with the 2 d administration group, the expression of age-1, daf-2, let-363 were significantly up-regulated (P < 0.01) in the 6 d administration group after treated with CXE while the expression of ins-18, let-60, sir-2.1, sod-3 were significantly down-regulated (P < 0.01).</p><p><b>CONCLUSION</b>In the progress of aging, the expression of age-1, daf-2, let-363 increased, functioning as aging-promoting genes; while the expression of ins-18, let-60, sir-2.1, sod-3 decreased, functioning as longevity genes; CXE extended the lifespan through inhibiting the expression of these aging-promoting genes and increasing the expression of longevity genes, which would be the molecular mechaniSm of anti-aging of traditional Chinese medicine that can promote Qi and activate blood.</p>
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Assunto principal:
Farmacologia
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Medicamentos de Ervas Chinesas
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Caenorhabditis elegans
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Regulação da Expressão Gênica no Desenvolvimento
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Proteínas de Caenorhabditis elegans
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Genética
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Longevidade
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Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Article