The effects of a hot water soluble extract (S-03) isolated from Isatis indigotica root on influenza A and B viruses in vitro / 病毒学报
Chinese Journal of Virology
; (6): 218-223, 2011.
Article
em Zh
| WPRIM
| ID: wpr-286051
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WPRO
ABSTRACT
This study was to investigate the antiviral effects of a hot water soluble extract S-03 isolated from Isatis indigotica root on different subtypes of influenza A and B viruses in MDCK cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (HAD) assays. Chemical analysis of the extract S-03 showed that it contained high proportion of polysaccharides. The antiviral effects in vitro showed that the S-03 had no effect on different influenza viruses if the drug was used before virus adsorption, but S-03 showed obvious activities against influenza viruses if treatment after virus adsorption or direct reaction of drug and virus before virus adsorption. Hemagglutination inhibition assay showed that S-03 inhibited HA activities of different human influenza viruses (inhibition concentration ranged from 3.12 to 25 mg/mL), avain influenza viruses (inhibition concentration ranged from 25 to 50 mg/mL). The antiviral effects of S-03 on different influenza A and B viruses in vitro might be through the inhibition of the HA to prevent infection.
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Índice:
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Assunto principal:
Vírus da Influenza A
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Vírus da Influenza B
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Farmacologia
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Testes de Inibição da Hemaglutinação
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Extratos Vegetais
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Células Cultivadas
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Química
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Imunofluorescência
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Raízes de Plantas
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Isatis
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2011
Tipo de documento:
Article