DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells / 生物工程学报
Chinese Journal of Biotechnology
; (12): 727-732, 2009.
Article
em Zh
| WPRIM
| ID: wpr-286650
Biblioteca responsável:
WPRO
ABSTRACT
DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase (DNA-PK), plays an important role in the nonhomologous end-joining (NHEJ) pathway of DNA double-strand breaks (DSBs) repair. To investigate the effects of DNA-PKcs down-regulation on cell growth and sensitization to low dose radiation (LDR), we transfected DNA-PKcs siRNA into human mammary epithelia cells MCF10F, then, detected the proliferation curve of the cells and the expression of protiens in DNA repair pathways. The results showed that DNA-PKcs gene silencing, induced by the transfection of DNA-PKcs siRNA could suppress significantly the cell proliferation and inhibit the expression of the DNA repair proteins, such as Ku80, ATM and P53 after 50 cGy 137Cs gamma-irradiation.The results suggested that DNA-PKcs gene silencing could increase the sensitivity of human breast epithelial cells to the LDR, which might be relative with the decrease of the proteins.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Efeitos da Radiação
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Mama
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Proteínas Nucleares
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Transfecção
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Linhagem Celular
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Inativação Gênica
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Biologia Celular
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RNA Interferente Pequeno
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Reparo do DNA
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Relação Dose-Resposta à Radiação
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Article