Determination of brucine and strychnine in rat after cutaneous administration of semen strychni niosome gel by LC-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 853-857, 2012.
Article
em Zh
| WPRIM
| ID: wpr-288691
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>A sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed and validated for the determination of brucine and strychnine in rat plasma.</p><p><b>METHOD</b>Samples were extracted by ethyl acetate-n-butanol (7 3). Chromatographic separation was operated on ZORBAX XDB-C18 column with gradient elution of acetonitrile-methanol-water (0.05% acetic acid and 10 nmol x L(-1) ammonium formate contained), followed by LC-MS/MS in positive electrospray ionization. Quantification was carried out on multiple reaction monitoring (MRM) of the transition m/z 395.2/324.2, m/z 335.2/184.2 and m/z 199.1/171.1 for brucine, strychnine and tacrine (internal standard), respectively.</p><p><b>RESULT</b>The method was linear in the range of 0.195-100 and 0.07840 microg x L(-1) for brucine and strychnine, with coefficient correlation 0.994 and 0.996 respectively. The recoveries of extraction were 78.9% - 102.4% for brucine and 95.2% - 106.1% for strychnine. Precision, accuracy, stability and matrix effect of the analytes met the requirement. The method was applied to a pharmacokinetic study of brucine and strychnine after cutaneous administration of Semen Strychni niosome gel. The C(max) were (26.20 +/- 5.81) and (12.50 +/- 3.00) microg x L(-1) while the AUC(0-infinity), were (193.75 +/- 39.43) and (98.25 +/- 28.54) microg x h x L(-1) of the two components.</p><p><b>CONCLUSION</b>We conclude that the niosomes may reduce the systemic exposures and prolong the local release of brucine and strychnine.</p>
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Índice:
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Assunto principal:
Plantas Medicinais
/
Sementes
/
Sêmen
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Estricnina
/
Administração Cutânea
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Farmacocinética
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Química
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Cromatografia Líquida
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Ratos Sprague-Dawley
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Organismos Livres de Patógenos Específicos
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Article