Analysis of characteristics of major pathogenicity-related genes of Vibrio cholerae isolated in Guangzhou area from 2001 to 2005 / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 257-261, 2006.
Article
em Zh
| WPRIM
| ID: wpr-290276
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To apply multiplex polymerase chain reaction (MPCR) assay and sequencing in study of the carrying status of four pathogenicity-related genes of Vibrio cholerae (V.cholerae) and the variation of ctxA.</p><p><b>METHODS</b>Primers targeting cholera toxin sub-unit A gene (ctxA), toxin-coregulated pilus gene (tcpA), accessory cholera enterotoxin gene (ace), zonula occludens toxin gene (zot) were designed and the MPCR method was applied to detect the pathogenicity-related genes of 276 strains of V.cholerae isolates. The amplified fragments of ctxA gene were sequenced and the genetic homology of the amplified fragments of ctxA was analyzed.</p><p><b>RESULTS</b>Of the 276 strains of V.cholerae, 93.9% strains from human sources belong to the pathogenicity-related genes type A (ctxA(+)tcpA(+)ace(+)zot(+) type) and 6.1% belong to pathogenicity-related genes type C (ctxA(-)tcpA(-)ace(-)zot(-) type). Type A strains from clinical sources were isolated from patients with mild to severe symptom and carriers, among which 68.5% were isolated from patients with mild symptom and 21.9% from carriers. All 63.6% of type C strains from clinical sources were isolated from patients with mild symptom and 36.4% from carriers. The proportion of type C strains that caused mild symptom was higher than that of type A strains. Of the 78 strains isolated from the environment, 9.0% strains belong to pathogenicity-related type A and 35.9% belong to the pathogenicity-related genes type B (ctxA(-)tcpA(-)ace(+)zot(+) type), while 55.1% belong to pathogenicity-related genes type C. The sequencing results showed little genetic variation among the amplified fragments for ctxA.</p><p><b>CONCLUSION</b>MPCR disclosed the polymorphic status of pathogenicity-related gene patterns in V.cholerae isolates of Guangzhou, providing effective means for further study on evolution of pathogenicity-related genes among V.cholerae isolates from human and environmental sources. This study also offers significant guidance for effective prevention, control and warning against cholera epidemic in local area.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Vibrio cholerae
/
DNA Bacteriano
/
China
/
Toxina da Cólera
/
Reação em Cadeia da Polimerase
/
Classificação
/
Análise de Sequência
/
Genes Bacterianos
/
Genética
/
Genótipo
Tipo de estudo:
Guideline
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2006
Tipo de documento:
Article