Research on the treatment of liver failure rats with transplantation of alginate microencapsulated hepatocytes in vivo based on poly-ornithine / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 642-647, 2014.
Article
em Zh
| WPRIM
| ID: wpr-290700
Biblioteca responsável:
WPRO
ABSTRACT
This study aims to explore the effects of alginate-poly ornithine-alginate (A-PLO-A) and barium alginate-poly ornithine-alginate (B-PLO-A) microcapsules as cells carriers during implantation. Mice hepatocytes coated in A-PLO-A and B-PLO-A microcapsules were implanted into rats with acute liver failure caused by intraperitoneal injection of D-galactosamine. The rat survival rate, liver cell growth, proliferation and metabolism within the microcapsules were investigated, as well as its effect on the improvement of rat acute liver failure. The influence of A-PLO-A-free microcapsules, B-PLO-A-free microcapsules, isolated liver cells, A-PLO-A microcapsule-coated and B-PLO-A microcapsule-coated liver cells was studied. It was found that the chemical-free microcapsules showed no positive effect on the rats with liver failures, with a death rate of 100% in both groups 3 days after the implantation. The ALT, AST and ALB levels were all improved in the isolated liver cell group, the A-PLO-A microcapsule-coated and the B-PLO-A microcapsule-coated groups. The survival rate of both microcapsule-coated liver cell groups was significantly higher than that of the chemical-free microcapsule group and the isolated liver cells group. The microcapsules were retrieved after 4 weeks' implantation, which were observed to be smooth with no cells attaching to the surface. No apparent fibrosis was observed. This research demonstrated the physical stability and the biocompatibility of the PLO-based alginate microcapsules and therefore they could be used as liver cell carriers during implantation.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Ornitina
/
Terapêutica
/
Transplante
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Falência Hepática
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Ácido Glucurônico
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Hepatócitos
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Alginatos
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Ácidos Hexurônicos
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2014
Tipo de documento:
Article