Screening and identification of genes associated with multi-drug resistance in colonic cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 388-391, 2012.
Article
em Zh
| WPRIM
| ID: wpr-290777
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify novel multi-drug resistance-related genes, and to explore the mechanisms of multi-drug resistance.</p><p><b>METHODS</b>Multi-drug resistant cell line Lovo/5-FU was established by incubation with increasing dose of 5-FU. The sensitivity to 5-FU and cis-diaminodichloroplatinum (CDDP) was measured by MTT assay. Two dimensional electrophoresis plus mass spectrum(2-DE/MS) was used to identify the differentially expressed protein between Lovo and Lovo/5-FU. The identified protein was then verified by Western blot analysis.</p><p><b>RESULTS</b>The IC50 concentrations of Lovo/5-FU to 5-FU and CDDP were increased by 31 and 3 times, compared with Lovo (both P<0.01). 2DE-MS showed that CAP-G and RhoGDI2 were up-regulated, whereas 6-PGL, DCI, Prdx-6 and Maspin were down-regulated in Lovo/5-FU. Western blot analysis confirmed that the expression levels of RhoGDI2 and CAP-G in Lovo/5-FU were increased by 6.14 and 2.98 fold respectively (both P<0.01), whereas Maspin was decreased to 5.2% of Lovo(P<0.01).</p><p><b>CONCLUSIONS</b>Multi-gene and multi-pathway are involved in the development of multi-drug resistance of colorectal cancer cells. CAP-G, RhoGDI2 and Maspin are potential multi-drug resistant genes.</p>
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Índice:
WPRIM
Assunto principal:
Proteínas Nucleares
/
Serpinas
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Neoplasias do Colo
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Resistência a Múltiplos Medicamentos
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Resistencia a Medicamentos Antineoplásicos
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Linhagem Celular Tumoral
/
Inibidor beta de Dissociação do Nucleotídeo Guanina rho
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Genética
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Proteínas dos Microfilamentos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2012
Tipo de documento:
Article