Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 120-125, 2009.
Article
em Zh
| WPRIM
| ID: wpr-294766
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.</p><p><b>METHODS</b>Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.</p><p><b>RESULTS</b>A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.</p><p><b>CONCLUSION</b>Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.</p>
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Índice:
WPRIM
Assunto principal:
RNA
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Transdução de Sinais
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Genoma Humano
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Estudo de Associação Genômica Ampla
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Genes
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Genética
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Genótipo
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Insuficiência Cardíaca
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2009
Tipo de documento:
Article