VEGF-induced tubulogenesis of endothelial cells from human brain malignant glioma in the three dimentional model / 中华病理学杂志
Chinese Journal of Pathology
; (12): 579-582, 2005.
Article
em Zh
| WPRIM
| ID: wpr-297287
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the tubulogenesis capability of malignant glioma-derived microvessel endothelial cells (GDMEC) from human brain with that of ECV304 cells in a three dimentional model and to explore the significance of GDMEC in the study on angiogenesis.</p><p><b>METHODS</b>The GDMEC were isolated from malignant gliomas of human brain and purified by selective binding to the monoclonal antibody against CD105 bound to the magnetic MACS MicroBeads. GDMEC and endothelial-like cell line ECV304 were compared with their capabilities of formatting tubule-like structure (TLS) in the three dimentional collagen matrix, with or without inducement by various concentration of vascular endothelial growth factor (VEGF).</p><p><b>RESULTS</b>The obtained GDMEC had a high purification (98%) and could be successfully cultured in vitro. GDMECs formed more TLS than ECV304 cells of the same number and at the same time points. VEGF could induce rapid formation of TLS in a dose-dependent manner, however, ECV304 cells were less response to VEGF stimulation.</p><p><b>CONCLUSIONS</b>GDMEC could maintain their endothelial characteristics and potential capability of angiogenesis. They were more response to VEGF than ECV304, therefore, more suitable for in vitro studies on tumor angiogenesis.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Neoplasias Encefálicas
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Endotélio Vascular
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Células Cultivadas
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Separação Imunomagnética
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Biologia Celular
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Células Endoteliais
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Fatores de Crescimento do Endotélio Vascular
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Relação Dose-Resposta a Droga
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Article