Nifedipine modifies plasma membrane functional integrity and inhibits osmosensitive calcium influx in human sperm / 中华男科学杂志
National Journal of Andrology
; (12): 428-430, 2007.
Article
em Zh
| WPRIM
| ID: wpr-297709
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of nifedipine of therapeutic dosage on the plasma membrane functional integrity and osmosensitive calcium influx in human sperm in vitro.</p><p><b>METHODS</b>Sperm samples were aseptically obtained from 10 healthy fertile men by masturbation and prepared by swim-up technique to produce a spermatozoal solution of high motility. The solution was then incubated with nifedipine of 20, 100 and 20 x 10(3) ng/ml respectively at 37 degrees C in vitro. The hypo-osmotic swelling (HOS) test was done to assess the sperm function. Intracellular calcium concentration was measured by fluorescent probe fura-2/AM before and after sperm medium dilution in distilled water.</p><p><b>RESULTS</b>The 20 x 103 ng/ml group showed significantly lower HOS scores than the control (P < 0.01). The 20, 100 and 20 x 10(3) ng/ml groups all showed significantly lower Ca2+ fluorescence D-value than the control (P < 0.01).</p><p><b>CONCLUSION</b>Nifedipine can modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm and affect male fertility in vitro in therapeutic dose.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Pressão Osmótica
/
Farmacologia
/
Fisiologia
/
Espermátides
/
Técnicas In Vitro
/
Nifedipino
/
Membrana Celular
/
Cálcio
/
Metabolismo
Limite:
Adult
/
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Article