Study of molecular mechanism of tanshinone II A inducing differentiation in acute promyelocytic leukemia NB4 cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2954-2958, 2008.
Article
em Zh
| WPRIM
| ID: wpr-298484
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate molecular mechanism of tanshinone II A inducing differentiation and apoptosis in acute promyelocytic leukemia NB4 cells.</p><p><b>METHOD</b>NB4 cells were cultured in vitro and treated with tanshinone II A and observed cellular morphology, cell category and the cellular proliferation. DNA microarray technique was used to analyze the gene expression profiles of NB4 cells induced by tanshinone II A.</p><p><b>RESULT</b>92.8% of NB4 cells treated with 0.5 mg x L(-1) tanshinone II A were induced into mature neutrophils, in which myetocytes and melamyetocytes were 27.0%, banded and segmented neutrophits 68.2%. Cell growth were inhibited. cDNA microarray showed the enormously expressed 183 genes including 23 differentiation associated genes, and other interrelated genes.</p><p><b>CONCLUSION</b>Tanshinone II A inducing differentiation in NB4 cells may be via regulation of many kinds of genes, especially differentiation associated genes expression. This partially explained the molecular mechanism of tanshinone II A inducing differentiation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Fenantrenos
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Medicamentos de Ervas Chinesas
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Leucemia Promielocítica Aguda
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Regulação Leucêmica da Expressão Gênica
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Diferenciação Celular
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Linhagem Celular Tumoral
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Abietanos
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Tratamento Farmacológico
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Article