Expression of hepatitis delta antigen Inner Mongolian strain in prokaryotic cell and analysis of its antigenicity / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 53-55, 2007.
Article
em Zh
| WPRIM
| ID: wpr-305497
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain high yield and good antigenic activity of HDV L-Ag and to detect different regional patients' sera to test the purified antigen's antigenicity.</p><p><b>METHODS</b>Hepatitis delta virus' sequence was obtained from Inner Mongolian patient by using RT-PCR and PCR methods, PET43a was used and His-tag was added at the HDV L-Ag 5' and 3' to construct the recombinant expression plasmid, transform the plasmid into host bacterium BL21 and induce it with IPTG. The expression supernatant was purified by saturated (NH4)2SO4 and affinity chromatography. The activity and antigenicity of the expressed product were analyzed by using EIA.</p><p><b>RESULTS</b>Comparison of results obtained with detection by using the expressed protein coated plate and ABBOTT Murex anti-Delta (total) of 15 positive and 10 negative sera, the consistency was good (100%).</p><p><b>CONCLUSION</b>EIA proved that the purified antigen had good antigenicity, no serological difference was found in detection between different region's sera, therefore the purified delta antigen may be useful in diagnostic and other research.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
/
Virologia
/
Sangue
/
Hepatite D
/
Proteínas Recombinantes
/
Dados de Sequência Molecular
/
Vírus Delta da Hepatite
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Expressão Gênica
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China
/
Sequência de Aminoácidos
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2007
Tipo de documento:
Article