Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 8-11, 2006.
Article
em Zh
| WPRIM
| ID: wpr-305558
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To study a new kind of adjuvant: transcutaneous immunization adjuvant.</p><p><b>METHODS</b>The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.</p><p><b>RESULTS</b>The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.</p><p><b>CONCLUSION</b>The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
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Toxinas Bacterianas
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Proteínas Recombinantes de Fusão
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Engenharia Genética
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Expressão Gênica
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Adjuvantes Imunológicos
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Western Blotting
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Cricetulus
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Células CHO
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Clonagem Molecular
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2006
Tipo de documento:
Article