Core promoter mutations of HBV isolated from patients with chronic hepatitis B in Guangxi / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 477-478, 2003.
Article
em Zh
| WPRIM
| ID: wpr-305886
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between HBV core promoter mutations and liver damage or HBeAg status.</p><p><b>METHODS</b>Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter in 59 sera from patients with chronic hepatitis B in Guangxi, then the HBV DNA positive products were sequenced by direct sequencing.</p><p><b>RESULTS</b>The HBV DNA positive rate of was 59.3%(35/59). All the patients were infected by mutants. The commonest mutation was the double mutation (A --> T at nt1762 and G --> A at nt1764), counting for 57.1% (20/35). The next was C --> G at nt1799, counting for 54.4% (19/35), but this was no function. A --> G at nt1752 (resulting in isoleucine to valine) was seen in 37.1% (13/35) of the HBV DNA positive patients, and T --> C at nt1753 was seen in 20% (7/35). The significant difference in the frequency of T1762A1764 mutant was found between HBeAg positive patients (31.3%) and negative patients (79.0%).</p><p><b>CONCLUSIONS</b>HBV core promoter mutations are common among patients with chronic hepatitis B in Guangxi. T1762A1764 mutant is associated with HBeAg status and chronic hepatitis.</p>
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Índice:
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Assunto principal:
Patologia
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Virologia
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Sangue
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Vírus da Hepatite B
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Regiões Promotoras Genéticas
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Mutação Puntual
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Hepatite B Crônica
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Genética
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Antígenos do Núcleo do Vírus da Hepatite B
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Antígenos E da Hepatite B
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Article