Functional regulation of endothelial Myosin light chain kinase in extravascular migration of fibrosarcoma cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 145-150, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310376
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the functional regulation of endothelial Myosin light chain kinase (MLCK) in extravascular migration of fibrosarcoma HT1080 cells.</p><p><b>METHODS</b>An in vitro model of fibrosarcoma cell transmigration across a monolayer of HUVEC cultured on collagen gel was applied to observe extravascular migration of HT1080 cells,and were the electrical resistance of HUVEC monolayer and endothelial MLC phosphorylation in extravascular migration of HT1080 cells.</p><p><b>RESULT</b>HT1080 cells migrated through endothelial cells into collagen gel, the electrical resistance of a HUVEC monolayer was reduced and endothelial MLC phosphorylation was enhanced in extravascular migration of fibrosarcoma cells. Endothelial MLCK inhibitor (ML-7) blocked extravascular migration of HT1080 cells and inhibited reduction of electrical resistance of a HUVEC monolayer and enhancement of endothelial MLC phosphorylation in extravascular migration of HT1080 cells in a dose-dependent manner.</p><p><b>CONCLUSION</b>Endothelial MLCK regulates fibrosarcoma cell transendothelial migration through MLC phosphorylation, leading to cytoskeletal reorganization and endothelial cell constriction, then fibrosarcoma cells migrate into extravascular tissue through the gaps between endothelial cells.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
/
Fisiologia
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Veias Umbilicais
/
Quinase de Cadeia Leve de Miosina
/
Movimento Celular
/
Biologia Celular
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Linhagem Celular Tumoral
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Células Endoteliais
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Fibrossarcoma
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Article