Lentinan-graft-polyethylenimine-a novel vector for gene delivery / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 39-45, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310393
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).</p><p><b>METHODS</b>The properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.</p><p><b>RESULT</b>The particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.</p><p><b>CONCLUSION</b>A novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Polietilenoimina
/
Terapia Genética
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Linhagem Celular
/
Química
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Técnicas de Transferência de Genes
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Ácido Fólico
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Lentinano
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Métodos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Article