Peptide CY11 conjugated polyethylenimine-beta-cyclodextrin for gene delivery / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 24-30, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310395
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel non-viral gene delivery vector CY11-PEI-beta-CyD and to test its gene transfection efficiency.</p><p><b>METHODS</b>CY11 (CGMQLPLATWY) was conjugated to polyethylenimine-beta-cyclodextrin to form CY11-PEI-beta-CyD with a cross-linker [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. (1)H-NMR and TGA were used to confirm the structure of vector. The DNA condensing ability of CY11-PEI-beta-CyD was investigated by gel retardation assay. Cytotoxicity of CY11-PEI-beta-CyD was determined by MTT assay and transfection efficiency was investigated in COS-7, Hela and B16 cells.</p><p><b>RESULT</b>CY11 was conjugated onto PEI-beta-CyD successfully, confirmed by(1)H NMR and TGA. The novel vector effectively condensed DNA at N/P ratio of 4îIt showed low cytotoxicity up to the concentration was 160 Mgr;g/ml. The transfection efficiency was 17-fold higher than that of PEI 25 kDa at N/P ratio of 20.</p><p><b>CONCLUSION</b>The novel vector CY11 -PEI-beta-CyD with low cytotoxic and high transfection efficiency may be used as a potential carrier for gene delivery.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fragmentos de Peptídeos
/
Polietilenoimina
/
Terapia Genética
/
Linhagem Celular
/
Química
/
Receptores de Fatores de Crescimento de Fibroblastos
/
Técnicas de Transferência de Genes
/
Beta-Ciclodextrinas
/
Métodos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Article