Assessment of heme oxygenase-1 (HO-1) activity in the cavernous tissues of sildenafil citrate-treated rats / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 377-381, 2007.
Article
em En
| WPRIM
| ID: wpr-310500
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To assess heme oxygenase-1 (HO-1) activity in the cavernous tissue of sildenafil citrate-treated rats.</p><p><b>METHODS</b>One hundred and ninety-two Sprague-Dawley male rats, divided into four equal groups, were investigated. Group 1, the control group, received regular animal chow; group 2 received sildenafil citrate by intragastric tube; group 3 received sildenafil and HO inhibitor (zinc protoporphyrin, ZnPP); and group 4 received sildenafil and nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester (L-NAME). Twelve rats from each group were killed after 0.5 h, 1 h, 2 h and 3 h of drug administration. Then HO-1 activity, cGMP levels and NOS enzymatic activity in the cavernous tissues were estimated.</p><p><b>RESULTS</b>In cavernous tissue, HO-1 activity, NOS enzymatic activity and cGMP concentration increased significantly in sildenafil-treated rats compared to other groups throughout the experiment. Rats receiving either HO or NOS inhibitors showed a significant decrease in these parameters. HO-1 cavernous tissue activity and NOS enzymatic activity demonstrated a positive significant correlation with cGMP levels (r = 0.646, r = 0.612 respectively; P < 0.001).</p><p><b>CONCLUSION</b>The actions of PDE5 inhibitor sildenafil citrate in the cavernous tissue are partly mediated through the interdependent relationship between both HO-1 and NOS activities.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Pênis
/
Farmacologia
/
Piperazinas
/
Protoporfirinas
/
Purinas
/
Sulfonas
/
Vasodilatadores
/
Administração Oral
/
Ratos Sprague-Dawley
/
GMP Cíclico
Limite:
Animals
Idioma:
En
Revista:
Asian Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Article