Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 373-376, 2009.
Article
em En
| WPRIM
| ID: wpr-311858
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).</p><p><b>METHODS</b>Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS.</p><p><b>RESULTS</b>After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P < 0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three biomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%.</p><p><b>CONCLUSIONS</b>SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sangue
/
Biomarcadores
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Diagnóstico
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Endometriose
/
Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2009
Tipo de documento:
Article