HPLC specific chromatogram of Dendrobium officinale / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the method of specific chromatogram analysis of ether extract of Dendrobium officinale for identification of D. officinale.</p><p><b>METHOD</b>Chromatographic separation was carried out at 30 degrees C on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) eluted with methanol and water containing 0.2% phosphoric acid in a gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of NPC (National Pharmacopoeia Committee) was adopted to specific chromatogram construction.</p><p><b>RESULT</b>The HPLC specific chromatogram of D. officinale was constructed with 6 common specific chromatographic peaks including naringenin as a reference peak.</p><p><b>CONCLUSION</b>The method shows good precision and repeatability of relative retention time. It can be used to identify D. officinale.</p>