Effect of liposomal transfection of antisense oligodeoxynucleotide on alpha-globin gene expression and proliferation of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1065-1069, 2007.
Article
em Zh
| WPRIM
| ID: wpr-318788
Biblioteca responsável:
WPRO
ABSTRACT
The objective of study was to investigate the effect of liposomal transfection of antisense oligodeoxynucleotide (ASON) on alpha-globin gene expression and proliferation of K562 cells, to explore the new way of gene therapy in beta-thalassemia. Targeted ASON of alpha-globin was designed and synthesized, and compared with positive control [sense oligodeoxynucleotide (SON) group] and blank control. By liposomal transfection, ASON, SON was co-cultured with K562. The efficiency of transfection was assayed by fluorescence microscopy and flow cytometry (FCM), the alpha-globin gene expression of K562 was measured by real-time PCR, and the proliferation of K562 was determined by Cell Count Kit-8 assay. The results indicated that the highest efficiency was at 24 hours after liposomal transfection, the gene expression level of alpha-globin in ASON group was significantly lower than that in SON group and blank control (p < 0.01). The proliferation of K562 cells was obviously inhibited, meanwhile the above effect showed the dose-dependent manner. It is concluded that the liposomal transfection of ASON inhibits the alpha-globin gene expression of K562 cells, which may be the new target for gene therapy in beta-thalassemia.
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Transfecção
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Expressão Gênica
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Oligonucleotídeos Antissenso
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Células K562
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Proliferação de Células
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Alfa-Globinas
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Lipossomos
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Article