Folate reduces monocyte chemoattractant protein-1 expression in rats with hyperhomocysteinemia / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 470-474, 2005.
Article
em Zh
| WPRIM
| ID: wpr-318882
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of supplementation of folate on the expression of monocyte chemoattractant protein-1 (MCP-1) in aortic endothelium and release from peripheral blood mononuclear cells (PBMC) in rats with hyperhomocysteinemia induced by ingestion of excess methionine.</p><p><b>METHODS</b>Thirty male SD rats were randomly divided into 3 groups (n = 10 for each group): control group (Control), high homocysteinemia group (Hhcy), and folate supplementation group (FA). They were fed with nomal diet, normal diet enriched by 1.7% methionine, and normal diet plus 1.7% methionine and 0.006% folate, respectively, for 45 days. The levels of total plasma homocysteine (Thcy) were measured by high performance liquid chromatography and the concentrations of chemokine MCP-1 released from PBMC stimulated by oxidized low density lipoprotein were detected by enzyme immunoassays. The expression of MCP-1 on aortas of rats was detected by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>A high methionine diet for 45 days induced hyperhomocysteinemia. Folate supplementation to high-methionine diet significantly decreased plasma Thcy levels (P < 0.01). The expression of MCP-1 in aortic endothelium and the levels of MCP-1 released from PBMC stimulated by oxidized low density lipoprotein were significantly higher in rats of Hhcy group than in rats of control group (P < 0.05, P < 0.01). During supplementation of folate, normalization of Thcy levels was accompanied by a marked reduction of MCP-1 expression in aortic endothelium and by a significant decrease of MCP-1 released from PBMC stimulated by oxidized low density lipoprotein (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Folate supplementation can prevent an elevation of homocysteine levels in the blood and decrease the expression MCP-1 in aortic endothelium and release of MCP-1 from PBMC in rats with hyperhomocysteinemia.</p>
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Índice:
WPRIM
Assunto principal:
Aorta
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Farmacologia
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Sangue
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Endotélio Vascular
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Leucócitos Mononucleares
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Distribuição Aleatória
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Ratos Sprague-Dawley
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Quimiocina CCL2
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Hiper-Homocisteinemia
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Biologia Celular
Limite:
Animals
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2005
Tipo de documento:
Article