Investigation of the expression of calcitonin receptor mRNA in human osteoclasts on deciduous teeth / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.</p><p><b>METHODS</b>After fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.</p><p><b>RESULTS</b>There were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.</p><p><b>CONCLUSION</b>On the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptor mRNA, so it is feasible to use this kind of osteoclast to test the effect of external factors on the expression of CTR mRNA.</p>