The effect of carbon monoxide on the proliferation of PASMCs under hypoxia and the mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 257-260, 2002.
Article
em Zh
| WPRIM
| ID: wpr-319314
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM AND METHODS</b>MTT colorimetric assay, in situ hybridization and immunocytochemistry were performed to investigate the effect of endogenous and exogenous CO on the proliferation of PASMCs and the expression of PDGF-B and protooncogene bcl-2, P53 (mutant type) in PASMCs, in order to elucidate the mechanism by which CO suppressed the proliferation of PASMC in hypoxic environment.</p><p><b>RESULTS</b>The results of in situ hybridization of PDGF-B mRNA and immunocytochemical staining of PDGF-B were negative. Hypoxia could upregulate the expression of Bcl-2, mutant P53 protein in comparison with the control group (P < 0.01). Compared with the hypoxic group, the expression of Bcl-2 and mutant P53 were decreased after treated with hemin or CO, but increased after treated with hemoglobin (P < 0.01).</p><p><b>CONCLUSION</b>CO could suppress the expression of oncogene bcl-2 and mutant P53. This partially explained how CO suppressed the proliferation of PASMCs in hypoxic environment.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Artéria Pulmonar
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Monóxido de Carbono
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Hipóxia Celular
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Células Cultivadas
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Ratos Wistar
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Biologia Celular
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Miócitos de Músculo Liso
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Proliferação de Células
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Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2002
Tipo de documento:
Article