Expressions of transient receptor potential A1 and related inflammatory factors in the rat model of prostatic inflammation / 中华男科学杂志
National Journal of Andrology
; (12): 23-30, 2015.
Article
em Zh
| WPRIM
| ID: wpr-319548
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanism of pain associated with chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) in the rat model of prostatic inflammation.</p><p><b>METHODS</b>Thirty-six male SD rats were equally randomized to an experimental and a control group, the former injected with 50 μl of 3% λ-carrageenan into the ventral prostate to make the model of non-bacterial prostatic inflammation, while the latter with the same volume of sterile saline solution. At 1, 2 and 4 weeks after modeling, the prostate, L6-S1 dorsal root ganglion (DRG) and spinal cord were harvested for examination of the expressions of the nerve growth factor (NGF), transient receptor potential ankyrin 1 (TRPA1), and calcitonin-gene-related peptide (CGRP) by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>The expressions of NGF, TRPA1 and CGRP in the prostatic tissue were all significantly increased in the experimental group as compared with the control (P <0.05), with a gradual decrease with the prolonging of time (P <0.05). In the L6-S1 DRG and spinal cord, the expressions of NGF, TRPA1 and CGRP exhibited no significant differences between the experimental and control groups at 1 week after modeling (P >0.05) and kept at high levels in the experimental group at 2 and 4 weeks, though not significantly different from those at 1 week (P >0.05). Statistically significant differences were observed in the expressions of the three proteins in the experimental rats among different time points (P <0.05), but not between the two groups at any time point (P >0.05).</p><p><b>CONCLUSION</b>The molecular mechanism of CP/CPPS can be evaluated in the rat model of prostatic inflammation established by injecting λ-carrageenan into the prostate. TRPA1 may play an important role in connecting the upstream and down-stream pathways of CP/CPPS-associated pain.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Prostatite
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Medula Espinal
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Carragenina
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Peptídeo Relacionado com Gene de Calcitonina
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Doença Crônica
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Ratos Sprague-Dawley
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Dor Pélvica
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Fator de Crescimento Neural
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Canais de Cátion TRPC
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Dor Crônica
Tipo de estudo:
Clinical_trials
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Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2015
Tipo de documento:
Article