Preparation of novel gypenosides long-circulating liposomes consisted by sphingomyelin and beta-sitosterol modified by PEG / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 997-1001, 2014.
Article
em Zh
| WPRIM
| ID: wpr-321379
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of preparing novel gypenosides long-circulating liposomes with PEG grafted on beta-sitosterol (PEG-Sito).</p><p><b>METHOD</b>Succinicanhydride was adopted to connect beta-sitosterol and PEG 2000. Sphingomyelin and PEG-Sito was used as material to prepare gypenosides long-circulating liposomes by using ethanol injection method. Encapsulation efficiency was determined by using protamine precipitation method. H-NMR was used to verify the synthesis of PEG-Sito, the novel gypenosides long-circulating liposomes were characterized by particle size, zeta potential and atomic force microscope.</p><p><b>RESULT</b>The synthesis of PEG-Sito was verified by 1H-NMR. Encapsulation efficiency of long-circulating liposomes prepared by ethanol injection method was 74.3%, particle size was 288.1 nm, zeta potential was -20.25 mV, the morphology were round observed by AFM.</p><p><b>CONCLUSION</b>The novel gypenosides long-circulating liposomes prepared with PEG-Sito was feasible, it had a high encapsulation efficiency and good morphology.</p>
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Índice:
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Assunto principal:
Polietilenoglicóis
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Sitosteroides
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Esfingomielinas
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Sangue
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Extratos Vegetais
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Química
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Estudos de Viabilidade
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Reprodutibilidade dos Testes
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Gynostemma
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Composição de Medicamentos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article