Study of vascular endothelial growth factor and transforming growth factor beta1 genes regulate the mineral-related genes in human cells from apical papilla / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 468-473, 2012.
Article
em Zh
| WPRIM
| ID: wpr-322357
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone the VEGF165 gene and to construct eucaryotic expression vector, investigate the effect of overexpressed VEGF165 and transforming growth factor beta1 (TGFbeta1) on the mineral-related genes in human cells from apical papilla.</p><p><b>METHODS</b>Total RNA of ECV304 cell was extracted. The VEGF165 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and then was subcloned into eucaryotic expression vector pcDNA3.1hisA to construct the recombinant vector pcDNA3.1hisA-VEGF165. After being identified by digestion and DNA sequencing, pcDNA3.1hisA-VEGF165, and pcDNA3.1hisA-TGFbeta1 were transfected into human cells from apical papilla Then the efficiency of gene transfection and the expression of bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP), osteocalcin (OCN), dentin matrix protein 1 (DMP1) were detected by Real-Time polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Cloned VEGF165 gene sequences and inserted into expression vector of the VEGF165 sequences showed 100% homology related to the sequence in GenBank database. VEGF165 and TGFbeta1 mRNA were upregulated after transfection. The expression of DSPP mRNA were significantly increased in each experiment group (P < 0.05). The expression of OCN mRNA were increased significantly in the group transfected with pcDNA3.1hisA-TGFbeta1 and transfected with two plasmids (P < 0.05). The expression of BSP mRNA were not varying (P > 0.05), while no expression of DMP1 mRNA in each experiment group.</p><p><b>CONCLUSION</b>The recombinant eucaryotic expression vector of pcDNA3.1hisA-VEGF165 was constructed successfully. VEGF165 and TGFbeta1 can induce the expression of most mineral-related genes and they may play a key role during the differentiation of human cells from apical papilla.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
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RNA Mensageiro
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Transfecção
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Osteocalcina
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Células Cultivadas
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Clonagem Molecular
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Fator A de Crescimento do Endotélio Vascular
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Fator de Crescimento Transformador beta1
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Vetores Genéticos
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Minerais
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2012
Tipo de documento:
Article