Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 14-16, 2008.
Article
em Zh
| WPRIM
| ID: wpr-325645
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).</p><p><b>METHODS</b>The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.</p><p><b>RESULTS</b>CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).</p><p><b>CONCLUSIONS</b>CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Reação em Cadeia da Polimerase
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Ciclinas
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Leucemia-Linfoma Linfoblástico de Células Precursoras
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Ciclina C
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Fluorescência
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Genética
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Metabolismo
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Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2008
Tipo de documento:
Article