Effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase on hepatocellular carcinoma in mice / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 276-279, 2010.
Article
em Zh
| WPRIM
| ID: wpr-326384
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.</p><p><b>METHODS</b>Kunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.</p><p><b>RESULTS</b>TK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P < 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P < 0.05; group D vs group C, P < 0.05; group D vs group B, P < 0.05).</p><p><b>CONCLUSION</b>Ultrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.</p>
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Assunto principal:
Terapêutica
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Timidina Quinase
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Ultrassom
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Terapia Genética
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Resultado do Tratamento
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Simplexvirus
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Carcinoma Hepatocelular
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Linhagem Celular Tumoral
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Microbolhas
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Genes Transgênicos Suicidas
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2010
Tipo de documento:
Article