Cytotoxicity of MICA-reactive V delta 1 gamma delta T cells towards epithelial tumor cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 1-7, 2004.
Article
em Zh
| WPRIM
| ID: wpr-326994
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To confirm whether human MHC class I chain-related A (MICA) induces the amplification of V delta 1 gamma delta tumor-infiltrating lymphocytes (TILs) in vitro and to identify the cytotoxicity of MICA-reactive V delta 1 gamma delta TILs towards epithelial tumor cells.</p><p><b>METHODS</b>MICA protein was prokaryoticly expressed and purified by molecular cloning technology. The purified recombined MICA (rMICA) was used to induce V delta 1 gamma delta T cells from tumor tissues in vitro and the cytotoxicity of these V delta 1 gamma delta TILs were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT).</p><p><b>RESULTS</b>The rMICA was expressed in prokaryocyte with pET30 as a vector. The immobilized rMICA protein could markedly induce the amplification of V delta 1 gamma delta T cells from tumor tissue in vitro. These V delta 1 gamma delta T cells showed strong cytolytic activities towards tumor cell lines expressing MICA.</p><p><b>CONCLUSION</b>The MICA-reactive V delta 1 gamma delta T cell may be a candidate for adoptive cellular therapy of tumors.</p>
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Índice:
WPRIM
Assunto principal:
Neoplasias Ovarianas
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Patologia
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Proteínas Recombinantes
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Células HeLa
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Linfócitos T Citotóxicos
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Antígenos de Histocompatibilidade Classe I
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Imunoterapia Adotiva
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Linfócitos do Interstício Tumoral
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Receptores de Antígenos de Linfócitos T gama-delta
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Alergia e Imunologia
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2004
Tipo de documento:
Article