An efficient tool for the construction of multiple-cistronic vectors: FMDV 2A / 生物工程学报
Chinese Journal of Biotechnology
; (12): 765-769, 2007.
Article
em Zh
| WPRIM
| ID: wpr-327950
Biblioteca responsável:
WPRO
ABSTRACT
Recently, cancer therapy with mutiple genes has been attached with great attention. However, at present there is no efficient tool to construct multiple-cistrons. The large sizes and the imbalance in expression of most traditional tools, such as ribosome entry sites (IRESes),greatly block their wide employment in the construction of multiple cistronic gene therapy vectors. The self-cleaving peptide 2A from foot-and-mouth disease virus (FMDV) has a very small size, and more importantly, high cleavage activity in artifical bicistron, which bring new hope for mutiple genes therapy stategy. In this article, the characteristics and cleavage activities of FMDV 2A will be elucidated,and we further outline its applications in cancer gene therapy.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Terapêutica
/
Fatores de Transcrição
/
DNA Recombinante
/
Dados de Sequência Molecular
/
Terapia Genética
/
Splicing de RNA
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Sequência de Aminoácidos
/
Regiões Promotoras Genéticas
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Fusão Gênica Artificial
/
Vírus da Febre Aftosa
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Article