Effects of Nanofiber Alignment and Strain Direction on Cellular Activities of Human ACL Fibroblasts / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
; : 1-12, 2004.
Article
em Ko
| WPRIM
| ID: wpr-32843
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The effects of fiber alignment and direction of mechanical strain on the ECM generation of human ACL fibroblast were assessed. MATERIALS AND METHODS: The aligned nanofiber was fabricated using electrospinning with a rotating target. The amounts of collagen on aligned and randomly oriented structures were compared. To evaluate the effect of strain direction, 5% uniaxial strain (0.2 Hz) was applied to fibroblasts seeded on parallel aligned, vertically aligned to the strain direction, and randomly oriented nanofiber sheets. The amounts of collagen produced were measured 2 days after halting the strain application. RESULTS: The fibroblasts on the aligned nanofiber were spindle-shaped and oriented in the direction of the fibers. Significantly more collagen (22.5+/-2.7 ug/ngDNA) was synthesized on the aligned nanofiber than the randomly oriented (14.5+/-3.2 ug/ngDNA). And the amounts of collagen produced were increased by 150% and 50% approximately with the longitudinal and perpendicular cyclic strain, respectively. CONCLUSION: The aligned nanofiber scaffold used in this study constitutes a promising base material for tissue-engineered ligament in that it provides a more biomimetic structure, including the preferable mechanical environment.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Colágeno
/
Biomimética
/
Nanofibras
/
Fibroblastos
/
Ligamentos
Limite:
Humans
Idioma:
Ko
Revista:
Journal of Korean Orthopaedic Research Society
Ano de publicação:
2004
Tipo de documento:
Article