Chitosan/PIK3CA siRNA nanoparticle-mediated PIK3CA gene interference decreases the invasive capacity of gastric cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1503-1506, 2014.
Article
em Zh
| WPRIM
| ID: wpr-329259
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma.</p><p><b>METHODS</b>Gastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated cells was assessed with Transwell assay.</p><p><b>RESULTS</b>PIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level of PIK3CA and significantly decreased the invasion of BGC823 cells.</p><p><b>CONCLUSION</b>PIK3CA gene interference mediated by PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Neoplasias Gástricas
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Fosfatidilinositol 3-Quinases
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RNA Interferente Pequeno
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Linhagem Celular Tumoral
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Quitosana
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Proliferação de Células
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Nanopartículas
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Classe I de Fosfatidilinositol 3-Quinases
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Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Article