Protection effects of acetylcholinesterase inhibitors on apoptosis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 458-460, 2008.
Article
em Zh
| WPRIM
| ID: wpr-332467
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether tacrine and donepezil can prevent apoptosis induced by Lipopolysaccharides.</p><p><b>METHODS</b>Phase-contrast microscopes was used to observe the morphological changes of Vero cells. Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apoptotic biochemical changes.</p><p><b>RESULTS</b>Vero cells treated with 400 microg/ml and 500 microg/ml Lipopolysaccharides exhibited cell apoptosis. 10 micromol/L tacrine provided protective effect to 500 microg/ml Lipopolysaccharides induced cell apoptosis measured by Phase-contrast microscopes, cell counting kit-8 and DNA fragment analyze. However, donepezil did not show any protective effect of the apoptosis induced by 500 microg/ml Lipopolysaccharides.</p><p><b>CONCLUSION</b>Lipopolysaccharides can induce apoptosis in Vero cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaccharides induced apoptosis in Vero cells.</p>
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Índice:
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Assunto principal:
Patologia
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Farmacologia
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Fisiologia
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Piperidinas
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Tacrina
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Células Vero
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Células HeLa
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Contagem de Células
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Sobrevivência Celular
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Chlorocebus aethiops
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Article