2-DG enhances TRAIL-induced apoptosis of leukemia HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 351-355, 2013.
Article
em Zh
| WPRIM
| ID: wpr-332781
Biblioteca responsável:
WPRO
ABSTRACT
This study was purposed to investigate the effects of 2-deoxy-D-glucose (2-DG) on sensitizing HL-60 cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and its possible mechanism. The proliferative inhibition of HL-60 cells treated with different concentrations of 2-DG and TRAIL was measured by MTT assay. The cells were treated with 2-DG, TRAIL, and 2-DG combined with TRAIL at the concentration < IC50 value, i.e. 10 mmol/L for 2-DG and 100 ng/ml for TRAIL. Apoptosis was analyzed by flow cytometry with PI staining; the expression of RIP1, GRP78, and PARP was analyzed by Western blot; the activity of caspase-3 was detected by special detection kit. The results showed that the combined treatment of HL-60 cells for 48 h induced an apoptotic rate of (45.1 ± 4.3)%, which was significantly higher than that of treated with 2-DG or TRAIL alone; at the same time, the combined treatment potentiated the expression of GRP78 and caspase-3 activity, and down-regulated the expression of RIP1. It is concluded that 2-DG can sensitize HL-60 cells to TRAIL-induced apoptosis, which may be correlated with excessive endoplasmic reticulum stress response, down-regulation of RIP1, and increase of caspase-3 activity.
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Proteínas de Ligação a RNA
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Apoptose
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Células HL-60
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Complexo de Proteínas Formadoras de Poros Nucleares
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Desoxiglucose
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Caspase 3
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Ligante Indutor de Apoptose Relacionado a TNF
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Proteínas de Choque Térmico
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2013
Tipo de documento:
Article