The method of inducing and establishing human pancreatic cancer cell sublines with radiation resistance / 中华外科杂志
Chinese Journal of Surgery
; (12): 1640-1643, 2006.
Article
em Zh
| WPRIM
| ID: wpr-334439
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance.</p><p><b>METHODS</b>Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1 (Cap), AsPC-1 (ASPC), P3, PANC-1 (Pan-1) and MIAPaCa-2 (MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.</p><p><b>RESULTS</b>Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation. Cell cycle analysis suggested that SW1990-R, ASPC-R, MIA-R, PAN-R and P3-R had lower G(2)/M and greater SF(2) (survival fraction after 2 Gy irradiation) compared with the parental cell lines.</p><p><b>CONCLUSIONS</b>The method of radiating cells step by step and repeatedly is viable to establish radio-resistant pancreatic cancer cell lines.</p>
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Índice:
WPRIM
Assunto principal:
Neoplasias Pancreáticas
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Patologia
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Efeitos da Radiação
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Tolerância a Radiação
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Ciclo Celular
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Sobrevivência Celular
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Técnicas de Cultura de Células
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Linhagem Celular Tumoral
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Proliferação de Células
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Forma Celular
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Ano de publicação:
2006
Tipo de documento:
Article