Association between H-ras and L-myc gene polymorphisms and susceptibility to colorectal cancer / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 15-20, 2012.
Article
em Zh
| WPRIM
| ID: wpr-335353
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the association between the polymorphisms of oncogenes H-ras and L-myc and colorectal cancer risk, and the interaction of those genes.</p><p><b>METHODS</b>The genotypes of H-ras and L-myc genes were determined by polymerase chain reaction-based restriction fragment length polymorphism analysis. Stratified analysis and logistic model were used to detect the gene-gene interaction. The gene-gene interaction was validated by multifactor dimensionality reduction (MDR) analysis.</p><p><b>RESULTS</b>The single SNP model showed that the polymorphisms of H-ras and L-myc genes were not significantly related with colorectal cancer risk (P > 0.05). Stratified analysis revealed that among the L-myc LS + SS genotype carriers, those with H-ras TC + CC genotype showed significantly increased risk of rectal cancer than those with TT genotype (OR = 1.81, P = 0.005). The positive interaction between L-myc and H-ras was detected by logistic regression model. The OR of the interaction effect was 2.74 (P = 0.024). This result was confirmed in the MDR model, with 54.83% testing balanced accuracy and 10/10 cross-validation consistency, and the model was still significant after the 1000 times permutation test (P = 0.001).</p><p><b>CONCLUSION</b>Our findings suggest that the polymorphism of H-ras and L-myc genes is not related to colorectal cancer risk, but there is a synergy between H-ras and L-myc polymorphisms in the development of rectal cancer.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Neoplasias Retais
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Polimorfismo de Fragmento de Restrição
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Neoplasias Colorretais
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Modelos Logísticos
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Reação em Cadeia da Polimerase
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Risco
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Inquéritos e Questionários
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Genes myc
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Genes ras
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Neoplasias do Colo
Tipo de estudo:
Etiology_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2012
Tipo de documento:
Article