Down-regulation of p73 correlates with high histological grade in Japanese with breast carcinomas / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 2275-2278, 2011.
Article
em En
| WPRIM
| ID: wpr-338558
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>p73, a homologue of p53, has been located at chromosome 1p36-33, a region of frequently observed loss of heterozygosity in breast cancers. The objective of the present study was to investigate the function of p73 in Japanese with breast cancers.</p><p><b>METHODS</b>Sixty Japanese patients with breast cancer were assessed by polymerase chain reaction single strand confirmation polymorphism analysis and direct sequencing to detect the p73 allele. p73 mRNA levels were also determined in 40 out of 60 patients by reverse-transcriptional polymerase chain reaction.</p><p><b>RESULTS</b>We analyzed the entire open reading frame of the p73 gene by polymerase chain reaction single strand confirmation polymorphism and sequencing, and failed to identify any mutations of p73 in the encoding regions detected. Loss of heterozygosity of p73 was infrequent and only found in 9% of breast carcinomas. We revealed a few polymorphisms with a frequency of 13% - 29%, which had been reported previously. Down-regulation of p73 mRNA expression was observed in tumor tissues in comparison to the normal breast tissues. A significant inverse correlation was found between p73 transcripts and high histological grade, suggesting that down-regulated p73 expression could be related to poor prognosis in those patients.</p><p><b>CONCLUSION</b>Our results suggest that p73 may serve as a tumor suppressor gene and its expression plays a role in tumorigenesis in Japanese patients with breast cancer.</p>
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Assunto principal:
Patologia
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Neoplasias da Mama
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Proteínas Nucleares
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Carcinoma
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Proteína Supressora de Tumor p53
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Perda de Heterozigosidade
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Proteínas Supressoras de Tumor
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Proteínas de Ligação a DNA
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Proteína Tumoral p73
Limite:
Female
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Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2011
Tipo de documento:
Article