Expression of Aurora-B in human glioma tissue and its significance / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the expression of Aurora-B in human glioma tissue and its significance.</p><p><b>METHODS</b>The total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.</p><p><b>RESULTS</b>Aurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.</p><p><b>CONCLUSION</b>Aurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.</p>